Biocontrol in Greenhouses

David B. Orr and James R. Baker
Department of Entomology, North Carolina State University

Biological control is a tool to be considered in constructing an integrated pest management scheme for protected crop production. In a complete integrated pest management program, diseases, weeds, and plant growth must be considered as well as insects and mites. But because we are entomologists, this discussion covers insect and mite pests of greenhouse crops only. With IPM, each crop must be considered individually. Some greenhouse crops such as tomato and poinsettia are especially suitable for biocontrol for several reasons. Tomatoes and poinsettias have relatively few insect and mite pests. They are not grown year round so there is a break in the production cycle. Biological control organisms can be used especially effectively for caterpillars, spider mites and whiteflies. Table 1 gives biological controls now commercially available. Table 2 is a list of pesticides that are compatbility with various natural enemies used for biological control in the greenhouse. Following the tables are recommended management practices that simplify the task of setting up a biocontrol program for a greenhouse crop and then there are some sources of natural enemies.

Table 1. Recommended Natural Enemies for Greenhouse Insect Pests

PEST

NATURAL ENEMY

RATE

UNIQUE CHARACTERS

Aphids

Aphid Lions Chrysoperla spp.

Preventative: 1-3/yd, Monthly, as needed
Control (low infest.): 2 to 5/yd, Bi-Weekly,

2 to 3 times
Control (mod. infest.): 4 to 8/yd, Bi-Weekly,

2 to 4 times
Control (high infest.): 7 to 12/yd, Bi-Weekly,

3 to 5 times
Maintenance: 1 to 2/yd, Tri-Weekly, Indefinitely
COMMENTS: Rates shown above for larvae. For eggs, multiply rate times 5; for adults, divide by 4

Purchase fed larvae that Are packaged individually. Lacewings are cannibalistic and should well dispersed.

Aphid midge
Aphidoletes
aphidomyza

Preventative: 1 to 3/yd, Monthly, as needed

Control (low infestation): 2 to 5/yd, Biwkly --

2 to 3 times
Control (mod. infest.): 4 to 7/yd, Weekly,

2 to 4 times
Control (high infestation): 6 to 9/yd, Weekly,

3 to 5 times
Maintenance: 2 to 3/yd, Monthly, Indefinitely
COMMENTS: Preventive releases should be made in sites open to the outside

Drop from plants into soil to pupate. If soil is not directly beneath foliage (i.e. if roots are bagged, etc.), they will die and not reproduce in the greenhouse. Can be kept active by using long-day lights.

Aphid Parasitoids
Aphidius
matricarae

Preventative: 1 to 2/ yd, Bi-weekly, as needed
Control (low infestation): 2 to 4/yd, weekly,

2 to 3 times
Control (mod. infestation): 4 to 8/yd, weekly,

2 to 4 times
Control (high infestation): N/A
Maintenance: 2 to 3/yd, monthly

Shipped as live adults, ready to parasitize aphids.

Lady beetle
Hippodamia
convergens


"Bug Pro" and other synthetic food sources are available to help keep lady in the garden.

Syrphid maggots


May occur naturally where screening is not employed.

Broad mite

Predaceous mite
Neoseiulus
barkeri

10 to 30 per plant.


Caterpillars

Aphid lions
Chrysoperla spp.


Purchase fed larvae that Are packaged individually. Lacewings are cannibalistic and should well dispersed.

Bacterium
Bacillus
thuringiensis
kurstaki (various formulations)

1 to 5 teaspoons per gallon of water

Can be used with regular chemical pesticides

Fungus gnats


Yellow sticky cards and potato slices useful for monitoring.

Bacterium
Bacillus
thuringiensis
israelinsis
(Gnatrol™)

2 to 8 pints per 100 gallons of water.

Compatible with most pesticides.

Nematodes
Steinernema
carpocapsae
(Scanmask™)

Steinernema
carpocapsae (Ecomask™)


19,000 per sq ft.



70,000 per sq ft.


This strain is adapted to cooler soil temperatures.

This strain is adapted to warmer soil temperatures.

Soil-Dwelling Mite
Hypoaspis
miles

Preventative: 35 to 70/yd, Monthly, as needed
Control (low infest.): 45 to 100/yd, Tri-Weekly,

2 to 3 times
Control (mod. infest.): 100 to 140/yd, Bi-Weekly,

2 to 4 times
Control (high infest.): 100 to 200/yd, Bi-Weekly,

3 to 5 times
Maintenance: 4 to 75/yd, Monthly, Indefinitely
COMMENTS: Interval depends upon site size

Will also feed on thrips pupae in the soil.

Leafminers

Warm Weather
Leaf Miner
Parasitoid
Dacnusa
siberica

Preventative: 1 to 3/yd, Bi-Weekly, as needed
Control (low infestation): 2 to 5/yd, Bi-Weekly,

2 to 3 times
Control (moderate infestation): N/A Control (high infestation): N/A
Maintenance: 2 to 4/yd, Monthly, Indefinitely
COMMENTS: Early season introduction will work the best. Use with parasitic nematodes


Warm Weather
Leaf Miner
Parasitoid
Diglyphus
isaea

Preventative: 1 to 2/yd, Tri-Weekly, as needed
Control (low infest.): 2 to 4/yd, Bi-Weekly,

2 to 3 times
Control (mod. infestation): N/A
Control (high infestation): N/A
Maintenance: 2 to 3/yd, Monthly, Indefinitely
COMMENTS: Late spring introductions will work the best. Use with parasitic nematodes


Mealybugs

Australian lady beetle
Cryptolaemus
montrouzieri

Preventative: N/A
Control (low infest.): 2 to 4/yd, Tri-Weekly,

2 to 3 times
Control (mod. infest.): 4 to 6/yd, Tri-Weekly,

2 to 3 times
Control (high infest.): 6 to 8/yd, Bi-Weekly,

2 to 4 times
Maintenance: 2 to 3/yd, Monthly, Indefinitely
COMMENTS: Large scale use is normally in the southern states.

Lady beetles are somewhat sensitive to pesticides including soap. Lady beetles work best at high mealybug populations and not so well with low infestations.

Parasitic wasp
Leptomastix
dactylopii

2 per sq yd or 5 per infested plant. Repeat 1 or 2 times per year.

Use in conjunction with the Australian lady beetle. Good control in 2 to 3 months. CITRUS MEALYBUG ONLY.

Mealybug,
Longtailed

Aphid Lions
Chrysoperla
carnea

Preventative: 1 to 3/yd, Monthly, as needed
Control (low infest.): 2 to 5/yd, Bi-Weekly,

2 to 3 times
Control (mod. infest.): 4 to 8/yd, Bi-Weekly,

2 to 4 times
Control (high infest.): 7 to 12/yd, Bi-Weekly,

3 to 5 times
Maintenance: 1 to 2/yd, Tri-Weekly, Indefinitely
COMMENTS: Rates shown above for larvae. For eggs, multiply rate times 5; for adults, divide by 4

Larvae are recommended.

Scale
insects

Lady beetles (Harmonia
axyridis: or
Hippodamia)

Preventative: N/A
Control (low infest.): 1 to 2/yd, Tri-Weekly,

2 to 3 times
Control (mod. infest.): 2 to 3/yd, Tri-Weekly,

2 to 3 times
Control (high infest.): 3 to 4/yd, Bi-Weekly,

3 to 4 times
Maintenance: 1-3/yd, Qtrly - Indefinitely
COMMENTS: The release rate shown above are suggested until further data is available


Soft scales

Parasitic wasp
Metaphycus
helvolus


5 to 10 per plant

For hemispherical scale, brown soft scale, nigra scale, black scale

Spider mites

Predaceous mites
Amblyseius
(= Neoseiulus)
fallacis

Preventative: 5 to 9/yd, Monthly, as needed
Control (low infest.): 10 to 18/yd, Tri-Weekly,

2 to 3 times
Control (mod. infest.): 18 to 26/yd, Bi-Weekly,

2 to 4 times
Control (high infest.): 26 to 32/yd, Bi-Weekly,

2 to 4 times
Maintenance: 3 to 6/yd, Monthly, Indefinitely
COMMENTS: Fall preventive releases should be made into groundcover only one time

Will also feed on thrips pupae in the soil.

Predaceous mites
Phytoseiulus
persimilis
Neoseiulus
cucumeris

Release all predaceous mites at a rate of 1 per sq ft of crop plus 10 per mite-infested leaf.


Thrips



No really effective biological control organism is available.

Insidious flower
bug
Orius insidiosus

Preventative: N/A
Control (low infest.): 2 to 3/yd, Tri-Weekly,

2 to 3 times
Control (mod. infest.): 3 to 4/yd, Bi-Weekly,

2 to 4 times
Control (high infest.): 4 to 6/yd, Weekly,

2 to 4 times Maintenance: 1 to 2/yd, Monthly, Indefinitely
COMMENTS: Use with Hypoaspis miles for mid- to long-term kill

Goes dormant with short days. Won't stay on plants not bearing pollen. Use in conjunction with Neoseiulus cucumeris.

Predatory
Mite
Amblyseius
(= Neoseiulus)
cucumeris

Preventative: 30 to 90./yd, Tri-Weekly, as needed
Control (low infest.): 90 to 150/yd, Weekly,

3 to 5 times
Control (mod. infest.): 150 to 210/yd, Weekly,

3 to 5 times
Control (high infest.): 210 to 270/yd, Weekly,

4 to 6 times
Maintenance: 60 to 120/yd, Monthly, Indefinitely
COMMENTS: The Preventative and Maintenance releases may be made with packets, repeated every 8 weeks


Soil-Dwelling Mite
Hypoaspis miles

Preventative: 70 to 90/yd, Monthly, as needed
Control (low infestation): 90 to 130/yd, Tri-Weekly,

2 to 3 times
Control (mod. infestation): 130 to 190/yd, Bi-Weekly,

2 to 4 times
Control (high infestation): 190 to 270/yd, Bi-Weekly,

3 to 5 times
Maintenance: 8 to 100/yd, Monthly, Indefinitely


Predaceous mite
Neoseiulus
cucumeris

10 to 200 per plant, then 10 to 50 per plant every 1 to 2 weeks


Predaceous wasp
Thripoctenus
americensis


Has been found to utilize the western flower thrips in Canada.

Whiteflies

Fungus
Beauveria
bassiana
(Naturalis-L™)


2 tsp to 2 tbs. per gallon of water


Apply 3 to 5 sprays for complete control.

The Greenhouse Whitefly Parasitoid
Encarsia
formosa

Preventative: 4 to 6/yd, Bi-Weekly, as needed
Control (low infestation): 7 to 9/yd, Weekly,

3 to 4 times
Control (mod. infest.): 10 to 14/yd, Weekly,

3 to 5 times
Control (high infestation): N/A
Maintenance: 5 to 7/yd, Monthly, Indefinitely
COMMENTS: For whitefly sensitive crops, try 125-150% of rates above plus Preventative: Weekly

Insecticidal soap can be used in conjunction with if applied in the "black scale" or "brown scale " stage.

The Mixed Species Whitefly Parasitoid
(Eretmocerus
californicus)

Preventative: 4 to 6/yd, Bi-Weekly, as needed
Control (low infestation): 7 to 9/yd, Weekly,

3 to 4 times
Control (mod. infest.): 10 to 14/yd, Weekly,

3 to 5 times
Control (high infestation): N/A
Maintenance: 5 to 7/yd, Monthly, Indefinitely
COMMENTS: For whitefly sensitive crops, try 125 to 150% of rates above plus Preventative: Weekly

Use twice as many as Encarsia formosa because this parasite has males.

Whitefly:
Silverleaf

The Mixed Species Whitefly Parasitoid
Eretmocerus
californicus

Preventative: 6 to 8/yd, Bi-Weekly, as needed
Control (low infestation): 9 to 13/yd, Weekly,

3 to 4 times
Control (mod. infest.): 14 to 18/yd, Weekly,

3 to 5 times
Control (high infestation): N/A
Maintenance: 7 to 9/yd, Tri-Weekly, Indefinitely
COMMENTS: Should you wish to experiment with outdoor releases, try 50 to 75% of rates above


Whitefly Destroyer
Delphastus
pusillus

Preventative: N/A
Control (low infest.): 1 to 2/yd, Tri-Weekly,

2 to 3 times
Control (mod. infest.): 2 to 3/yd, Bi-Weekly,

2 to 4 times
Control (high infestation): 3 to 4/yd, Weekly,

3 to 5 times
Maintenance: 1 to 2/yd, Monthly, Indefinitely
COMMENTS: For releases in tomatoes, use 150-200% of rates determined above


Release at "hot spots."

Some of the information in the chart above was provided by The Green Spot, Nottingham, NH

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